Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
Read more
Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.55328 | 0.377944 | 4.62579 | 0.519156 | |
| 10day-seedling | 3.49769 | 0.654844 | 5.30584 | 0.514287 | |
| 14day-seedling | 2.78658 | 0.296337 | 3.95927 | 0.809354 | |
| 25day-aerial | 3.40904 | 0.213372 | 5.17486 | 0.114892 | |
| 35day-aerial | 3.57028 | 0.544685 | 5.4109 | 0.404154 | |
| 42day-rosette | 3.54335 | 0.251976 | 5.24186 | 0.230963 | 42day-stem | 3.90777 | 0.214658 | 5.62333 | 0.365421 |
| 53day-rosette | 3.99737 | 0.463701 | 5.83988 | 0.405489 | 53day-stem | 4.07072 | 0.808675 | 5.70826 | 0.353087 | 53day-inflores | 4.29645 | 0.200943 | 5.76079 | 0.105622 | 53day-silique | 3.5156 | 0.442544 | 4.6582 | 0.468881 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 32.25 | 8.4 | 2.97e-3 | -2.53 | 8.31 | 3.16e-3 | -2.5 |
| 2 | 1.000 | 37.21 | 9.23 | 1.67e-3 | -2.78 | 9.36 | 1.52e-3 | -2.82 | |
| 3 | 1.000 | 31.03 | 6.76 | 9.23e-3 | -2.04 | 5.99 | 1.57e-2 | -1.8 | |
| 10day-seedling | 1 | 1.000 | 22.01 | 9.15 | 1.76e-3 | -2.76 | 3.66 | 7.91e-2 | -1.1 |
| 2 | 1.000 | 25.78 | 12.54 | 1.68e-4 | -3.77 | 6.72 | 9.47e-3 | -2.02 | |
| 3 | 1.000 | 24.58 | 13.21 | 1.05e-4 | -3.98 | 6.5 | 1.10e-2 | -1.96 | |
| 14day-seedling | 1 | 1.000 | 27.28 | 13.71 | 7.46e-5 | -4.13 | 7.07 | 7.46e-3 | -2.13 |
| 2 | 0.966 | 33.2 | 15.09 | 2.86e-5 | -4.54 | 12.19 | 2.14e-4 | -3.67 | |
| 3 | 0.999 | 30.98 | 13.19 | 1.07e-4 | -3.97 | 11.05 | 4.72e-4 | -3.33 | |
| 25day-aerial | 1 | 0.999 | 24.35 | 11.5 | 3.46e-4 | -3.46 | 5.74 | 1.88e-2 | -1.73 |
| 2 | 1.000 | 26.77 | 12.73 | 1.48e-4 | -3.83 | 6.48 | 1.12e-2 | -1.95 | |
| 3 | 1.000 | 25.41 | 11.53 | 3.37e-4 | -3.47 | 5.97 | 1.59e-2 | -1.8 | |
| 35day-aerial | 1 | 1.000 | 23.84 | 9.39 | 1.49e-3 | -2.83 | 3.85 | 6.92e-2 | -1.16 |
| 2 | 0.999 | 25.37 | 12.65 | 1.55e-4 | -3.81 | 6.52 | 1.09e-2 | -1.96 | |
| 3 | 0.985 | 24.85 | 11.65 | 3.11e-4 | -3.51 | 5.46 | 2.26e-2 | -1.65 | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 26.46 | 12.07 | 2.32e-4 | -3.63 | 6.38 | 1.20e-2 | -1.92 | |
| 3 | 1.000 | 24.39 | 10.87 | 5.36e-4 | -3.27 | 5.3 | 2.54e-2 | -1.59 | |
| 42day-stem | 1 | 0.997 | 22.56 | 1.87 | 5.36e-1 | -3.27 | 5.56 | 2.12e-1 | -1.67 |
| 2 | 0.999 | 23.01 | 1.47 | 7.58e-1 | -3.15 | 4.92 | 3.34e-1 | -1.49 | |
| 3 | 0.993 | 22.7 | 9.32 | 1.57e-1 | -2.85 | 3.12 | 1.24e-1 | -0.97 | |
| 53day-rosette | 1 | 1.000 | 22.34 | 8.29 | 3.20e-3 | -2.49 | 2.3 | 2.03e-1 | -0.69 |
| 2 | 1.000 | 22.62 | 10.33 | 7.77e-4 | -3.11 | 4.61 | 4.08e-2 | -1.39 | |
| 3 | 1.000 | 24.2 | 11.31 | 3.95e-4 | -3.4 | 4.65 | 3.98e-2 | -1.4 | |
| 53day-stem | 1 | 1.000 | 23.18 | 9.76 | 1.15e-3 | -2.94 | 3.24 | 1.06e-1 | -0.98 |
| 2 | 0.997 | 24.98 | 10.21 | 8.45e-4 | -3.07 | 5.56 | 2.12e-2 | -1.67 | |
| 3 | 1.000 | 24.64 | 7.03 | 7.65e-3 | -2.12 | 4.08 | 5.93e-2 | -1.23 | |
| 53day-inflores | 1 | 0.999 | 22.73 | 9.33 | 1.55e-3 | -2.81 | 4.18 | 5.52e-2 | -1.26 |
| 2 | 1.000 | 22.92 | 8.29 | 3.19e-3 | -2.5 | 3.74 | 7.49e-2 | -1.13 | |
| 3 | 0.998 | 22.85 | 9.02 | 1.92e-3 | -2.72 | 4.43 | 4.64e-2 | -1.33 | |
| 53day-silique | 1 | 1.000 | 30.23 | 10.39 | 7.46e-4 | -3.13 | 6.44 | 1.15e-2 | -1.94 |
| 2 | 1.000 | 30.41 | 11.12 | 4.50e-4 | -3.35 | 7.41 | 5.87e-3 | -2.23 | |
| 3 | 1.000 | 32.19 | 13.22 | 1.05e-4 | -3.98 | 9.49 | 1.39e-3 | -2.86 | |
NGS expression data
Expression of miR160a transcript in NGS dataSequences
miRNA transcript
GUAUGCCUGGCUCCCUGUAUGCCAUAUGCUGAGCCCAUCGAGUAUCGAUGACCUCCGUGGAUGGCGUAUGAGGAGCCAUGCAUAUCCUCAUACAUAUAUAAUUAUGUUUUGGUACCAAUUGAAUUGUGUUGAUGAGCUUGUUUUGUAGAAGUCUCUGUUCAUUGCCUCAUCAUGUGUAGAAGAGAUUAGAGGUACCUCACGAAUCCAUCAUAUGCUUUUUUCUUGUGACUUCCCAUAUAUUCCUUUGUUAGAUA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCUGGCUCCCUGUAUGCCA | miRNA | 4 | 24 | |
ATCGATGACCTCCGTGGAT | primer | 5p | 44 | 62 |
ACCTCTAATCTCTTCTACACATGATGA | primer | 3p | 167 | 193 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR160a found in PubMed |