Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 2.35223 | 0.646271 | 2.42474 | 0.513584 | |
| 10day-seedling | 0.01702 | 0.444279 | 1.82518 | 0.479803 | |
| 14day-seedling | 1.05194 | 0.827927 | 2.48926 | 0.0318315 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | 0.34506 | 0.270635 | 1.92928 | 0.471786 | |
| 42day-rosette | 0.47761 | 0.883501 | 2.31872 | 1.1038 | 42day-stem | 0.88952 | 0.530508 | 2.32434 | 0.37897 |
| 53day-rosette | 1.15408 | 0.405907 | 2.99658 | 0.319479 | 53day-stem | 0.71629 | 0.379362 | 2.15969 | 0.145673 | 53day-inflores | 0.61106 | 0.615686 | 2.07101 | 0.726171 | 53day-silique | 2.30377 | 0 | 3.42719 | 0 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 39.37 | 15.52 | 2.13e-5 | -4.67 | 15.43 | 2.26e-5 | -4.65 |
| 2 | 1.000 | 41.23 | 13.25 | 1.02e-4 | -3.99 | 13.39 | 9.33e-5 | -4.03 | |
| 3 | 1.000 | 41.82 | 17.55 | 5.22e-6 | -5.28 | 16.78 | 8.91e-6 | -5.05 | |
| 10day-seedling | 1 | 1.000 | 37.25 | 24.39 | 4.56e-8 | -7.34 | 18.89 | 2.06e-6 | -5.69 |
| 2 | 1.000 | 34.79 | 21.55 | 3.27e-7 | -6.49 | 15.73 | 1.84e-5 | -4.74 | |
| 3 | 1.000 | 35.03 | 23.66 | 7.55e-8 | -7.12 | 16.95 | 7.91e-6 | -5.1 | |
| 14day-seedling | 1 | 1.000 | 35.28 | 21.7 | 2.93e-7 | -6.53 | 15.06 | 2.93e-5 | -4.53 |
| 2 | 0.994 | 35.93 | 17.81 | 4.34e-6 | -5.36 | 14.91 | 3.25e-5 | -4.49 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | 0.998 | 35.54 | 21.08 | 4.50e-7 | -6.35 | 15.55 | 2.09e-5 | -4.68 |
| 2 | 0.998 | 35.48 | 22.76 | 1.41e-7 | -6.85 | 16.63 | 9.86e-6 | -5.01 | |
| 3 | 1.000 | 35.67 | 22.48 | 1.71e-7 | -6.77 | 16.29 | 1.25e-5 | -4.9 | |
| 42day-rosette | 1 | 1.000 | 32.54 | 19 | 1.91e-6 | -5.72 | 11.89 | 2.63e-4 | -3.58 |
| 2 | 0.995 | 35.57 | 21.19 | 4.18e-7 | -6.38 | 15.5 | 2.16e-5 | -4.67 | |
| 3 | 0.000 | 38.33 | 24.81 | 3.40e-8 | -7.47 | 19.24 | 1.61e-6 | -5.79 | |
| 42day-stem | 1 | 0.997 | 31.1 | 19.41 | 1.43e-6 | -5.84 | 14.12 | 5.69e-1 | -4.25 |
| 2 | 1.000 | 31.7 | 19.16 | 1.71e-1 | -5.77 | 13.66 | 8.19e-1 | -4.96 | |
| 3 | 1.000 | 35.71 | 22.33 | 1.90e-7 | -6.72 | 16.22 | 1.54e-1 | -4.82 | |
| 53day-rosette | 1 | 1.000 | 32.14 | 18.09 | 3.58e-6 | -5.45 | 12.1 | 2.27e-4 | -3.64 |
| 2 | 1.000 | 31.66 | 19.38 | 1.47e-6 | -5.83 | 13.66 | 7.72e-5 | -4.11 | |
| 3 | 1.000 | 33.68 | 20.79 | 5.52e-7 | -6.26 | 14.13 | 5.57e-5 | -4.25 | |
| 53day-stem | 1 | 0.999 | 35.46 | 22.04 | 2.32e-7 | -6.63 | 15.52 | 2.13e-5 | -4.67 |
| 2 | 0.996 | 35.8 | 21.03 | 4.67e-7 | -6.33 | 16.38 | 1.18e-5 | -4.93 | |
| 3 | 1.000 | 36.9 | 19.29 | 1.56e-6 | -5.81 | 16.34 | 1.21e-5 | -4.92 | |
| 53day-inflores | 1 | 0.998 | 33.17 | 19.78 | 1.11e-6 | -5.95 | 14.62 | 3.97e-5 | -4.4 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.997 | 36.5 | 22.67 | 1.50e-7 | -6.82 | 18.08 | 3.61e-6 | -5.44 | |
| 53day-silique | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 34.57 | 15.6 | 2.01e-5 | -4.7 | 11.87 | 2.67e-4 | -3.57 | |
NGS expression data
Expression of miR833 transcript in NGS dataSequences
miRNA transcript
UUUGGACGUAGAUGGUACUCUUACAGAUGGUGAUCGUGAAAACAAACGGAGGAGUUUGUUUGUUGUACUCGGUCUAGUUCAAACCAAAACAUCUUUGCAAAUAUGUAUGGUUUGAGCUAGACCGAUGUCAACAAACAAGCUGCUUCG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
TTGGACGTAGATGGTACTCTTACAGATG | primer | 5p | 2 | 29 |
CATCGGTCTAGCTCAAACCATACATA | primer | 3p | 102 | 127 |
UGUUUGUUGUACUCGGUCUAGU | sRNA | 5p | 57 | 78 |
UAGACCGAUGUCAACAAACAAG | sRNA | 3p | 118 | 139 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR833 found in PubMed |