Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.23703 | 1.16771 | 5.33271 | 0.975453 | |
| 10day-seedling | 5.75158 | 1.2224 | 7.55974 | 1.08109 | |
| 14day-seedling | 4.43388 | 0.818157 | 5.87143 | 0.0216992 | |
| 25day-aerial | 2.20625 | 0.53762 | 3.96879 | 0.578045 | |
| 35day-aerial | 2.10283 | 0 | 4.05276 | 0 | |
| 42day-rosette | 2.55174 | 1.09261 | 4.59946 | 0.540476 | 42day-stem | 2.20595 | 0.739213 | 3.92586 | 0.731676 |
| 53day-rosette | 2.14673 | 0.310784 | 3.90851 | 0.252673 | 53day-stem | 3.31811 | 0.997318 | 4.73481 | 0.761112 | 53day-inflores | 2.90037 | 0.5626 | 4.24974 | 0.435471 | 53day-silique | 4.65683 | 1.07852 | 5.79943 | 1.11854 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 37.74 | 9.76 | 1.16e-3 | -2.94 | 9.89 | 1.05e-3 | -2.98 | |
| 3 | 1.000 | 39.52 | 15.24 | 2.58e-5 | -4.59 | 14.47 | 4.40e-5 | -4.36 | |
| 10day-seedling | 1 | 1.000 | 18.99 | 6.13 | 1.43e-2 | -1.85 | 0.64 | 6.43e-1 | -0.19 |
| 2 | 1.000 | 25.91 | 12.67 | 1.54e-4 | -3.81 | 6.85 | 8.65e-3 | -2.06 | |
| 3 | 1.000 | 24.94 | 13.58 | 8.19e-5 | -4.09 | 6.86 | 8.59e-3 | -2.07 | |
| 14day-seedling | 1 | 1.000 | 30.66 | 17.09 | 7.18e-6 | -5.14 | 10.44 | 7.18e-4 | -3.14 |
| 2 | 0.971 | 31.36 | 13.25 | 1.03e-4 | -3.99 | 10.34 | 7.71e-4 | -3.11 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | 1.000 | 37.48 | 24.63 | 3.86e-8 | -7.41 | 18.87 | 2.09e-6 | -5.68 |
| 2 | 1.000 | 35.47 | 21.42 | 3.56e-7 | -6.45 | 15.18 | 2.69e-5 | -4.57 | |
| 3 | 0.999 | 35.54 | 21.66 | 3.03e-7 | -6.52 | 16.09 | 1.43e-5 | -4.84 | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.997 | 35.81 | 22.62 | 1.55e-7 | -6.81 | 16.43 | 1.13e-5 | -4.95 | |
| 42day-rosette | 1 | 0.998 | 37.01 | 23.48 | 8.57e-8 | -7.07 | 16.37 | 1.18e-5 | -4.93 |
| 2 | 1.000 | 32.86 | 18.47 | 2.75e-6 | -5.56 | 12.78 | 1.42e-4 | -3.85 | |
| 3 | 0.999 | 33.79 | 20.27 | 7.92e-7 | -6.1 | 14.7 | 3.76e-5 | -4.43 | |
| 42day-stem | 1 | 1.000 | 35.99 | 24.3 | 4.84e-8 | -7.31 | 18.99 | 1.92e-6 | -5.72 |
| 2 | 0.997 | 32.3 | 19.76 | 1.13e-1 | -5.95 | 14.3 | 5.28e-1 | -4.28 | |
| 3 | 1.000 | 37.03 | 23.65 | 7.60e-8 | -7.12 | 17.34 | 6.14e-6 | -5.23 | |
| 53day-rosette | 1 | 1.000 | 37.55 | 23.5 | 8.45e-8 | -7.07 | 17.51 | 5.37e-6 | -5.27 |
| 2 | 1.000 | 34.32 | 22.04 | 2.33e-7 | -6.63 | 16.32 | 1.22e-5 | -4.91 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | 0.999 | 33.13 | 19.71 | 1.17e-6 | -5.93 | 13.19 | 1.07e-4 | -3.97 |
| 2 | 0.997 | 36.47 | 21.69 | 2.95e-7 | -6.53 | 17.04 | 7.43e-6 | -5.13 | |
| 3 | 1.000 | 32.83 | 15.22 | 2.61e-5 | -4.58 | 12.27 | 2.02e-4 | -3.69 | |
| 53day-inflores | 1 | 0.999 | 33.95 | 20.55 | 6.49e-7 | -6.19 | 15.4 | 2.31e-5 | -4.64 |
| 2 | 0.999 | 32.6 | 17.97 | 3.89e-6 | -5.41 | 13.42 | 9.16e-5 | -4.04 | |
| 3 | 0.997 | 35.8 | 21.97 | 2.44e-7 | -6.61 | 17.38 | 5.87e-6 | -5.23 | |
| 53day-silique | 1 | 1.000 | 30.29 | 10.44 | 7.18e-4 | -3.14 | 6.49 | 1.11e-2 | -1.95 |
| 2 | 1.000 | 36.68 | 17.39 | 5.84e-6 | -5.23 | 13.68 | 7.61e-5 | -4.12 | |
| 3 | 1.000 | 34.42 | 15.45 | 2.23e-5 | -4.65 | 11.72 | 2.96e-4 | -3.53 | |
NGS expression data
Expression of miR399d transcript in NGS dataSequences
miRNA transcript
GGUUGGAUUACUGGGCGAAUACUCCUAUGGCAGAUCGCAUUGGCUAGAUAUGCAAGUAAAAUGCUUCUCUGCCAAAGGAGAUUUGCCCCGCAAUUCAUCC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCAAAGGAGAUUUGCCCCG | miRNA | 70 | 90 | |
AATACTCCTATGGCAGATCGCATTGG | primer | 5p | 18 | 43 |
TCCTTTGGCAGAGAAGCATTTTACTTG | primer | 3p | 53 | 79 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR399d found in PubMed |