Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.74735 | 0.516585 | 5.81986 | 0.403414 | |
| 10day-seedling | 4.95906 | 0.734932 | 6.76721 | 0.544991 | |
| 14day-seedling | 5.03331 | 0.405937 | 5.99491 | 1.08777 | |
| 25day-aerial | 4.94201 | 1.19716 | 6.71543 | 1.21386 | |
| 35day-aerial | 5.21794 | 0.428197 | 7.01215 | 0.348797 | |
| 42day-rosette | 5.05326 | 0.726754 | 6.8957 | 0.594871 | 42day-stem | 4.69192 | 0.351864 | 6.37393 | 0.427941 |
| 53day-rosette | 5.52195 | 0.416017 | 7.36445 | 0.280176 | 53day-stem | 4.58842 | 0.922585 | 6.27283 | 0.77146 | 53day-inflores | 4.77288 | 0.40141 | 6.00549 | 0.628181 | 53day-silique | 6.20043 | 0.283495 | 7.34302 | 0.312516 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 35.19 | 11.34 | 3.87e-4 | -3.41 | 11.25 | 4.11e-4 | -3.39 |
| 2 | 1.000 | 36.86 | 8.89 | 2.11e-3 | -2.68 | 9.02 | 1.93e-3 | -2.72 | |
| 3 | 1.000 | 36.47 | 12.19 | 2.14e-4 | -3.67 | 11.42 | 3.64e-4 | -3.44 | |
| 10day-seedling | 1 | 1.000 | 24.21 | 11.35 | 3.84e-4 | -3.42 | 5.85 | 1.73e-2 | -1.76 |
| 2 | 1.000 | 26.06 | 12.81 | 1.39e-4 | -3.86 | 7 | 7.83e-3 | -2.11 | |
| 3 | 1.000 | 27.48 | 16.11 | 1.41e-5 | -4.85 | 9.4 | 1.48e-3 | -2.83 | |
| 14day-seedling | 1 | 0.999 | 26.03 | 12.46 | 1.77e-4 | -3.75 | 5.82 | 1.77e-2 | -1.75 |
| 2 | 0.995 | 32.84 | 14.73 | 3.67e-5 | -4.43 | 11.83 | 2.75e-4 | -3.56 | |
| 3 | 0.988 | 30.13 | 12.34 | 1.93e-4 | -3.71 | 10.2 | 8.52e-4 | -3.07 | |
| 25day-aerial | 1 | 0.995 | 30.81 | 17.95 | 3.95e-6 | -5.4 | 12.19 | 2.14e-4 | -3.67 |
| 2 | 0.996 | 24.99 | 10.94 | 5.07e-4 | -3.29 | 4.7 | 3.84e-2 | -1.42 | |
| 3 | 0.998 | 25.31 | 11.43 | 3.62e-4 | -3.44 | 5.87 | 1.71e-2 | -1.77 | |
| 35day-aerial | 1 | 0.994 | 25.64 | 11.18 | 4.30e-4 | -3.37 | 5.65 | 2.00e-2 | -1.7 |
| 2 | 1.000 | 26.75 | 14.02 | 6.00e-5 | -4.22 | 7.89 | 4.21e-3 | -2.38 | |
| 3 | 1.000 | 25.65 | 12.46 | 1.78e-4 | -3.75 | 6.27 | 1.29e-2 | -1.89 | |
| 42day-rosette | 1 | 1.000 | 28.28 | 14.75 | 3.64e-5 | -4.44 | 7.64 | 5.01e-3 | -2.3 |
| 2 | 0.998 | 28.63 | 14.25 | 5.14e-5 | -4.29 | 8.56 | 2.65e-3 | -2.58 | |
| 3 | 1.000 | 23.86 | 10.34 | 7.73e-4 | -3.11 | 4.77 | 3.66e-2 | -1.44 | |
| 42day-stem | 1 | 0.999 | 26.39 | 14.72 | 3.75e-1 | -4.43 | 9.39 | 1.49e-1 | -2.83 |
| 2 | 0.998 | 27.78 | 15.23 | 2.59e-1 | -4.59 | 9.69 | 1.21e-1 | -2.92 | |
| 3 | 0.998 | 26.38 | 13 | 1.22e-1 | -3.91 | 6.69 | 9.69e-1 | -2.13 | |
| 53day-rosette | 1 | 1.000 | 24.78 | 10.74 | 5.87e-4 | -3.23 | 4.75 | 3.73e-2 | -1.43 |
| 2 | 1.000 | 23.06 | 10.78 | 5.70e-4 | -3.24 | 5.06 | 3.00e-2 | -1.52 | |
| 3 | 1.000 | 26.04 | 13.15 | 1.10e-4 | -3.96 | 6.49 | 1.11e-2 | -1.95 | |
| 53day-stem | 1 | 1.000 | 27 | 13.58 | 8.16e-5 | -4.09 | 7.06 | 7.49e-3 | -2.13 |
| 2 | 1.000 | 31.38 | 16.6 | 1.01e-5 | -5 | 11.95 | 2.53e-4 | -3.6 | |
| 3 | 1.000 | 28.73 | 11.12 | 4.49e-4 | -3.35 | 8.17 | 3.48e-3 | -2.46 | |
| 53day-inflores | 1 | 0.997 | 28.74 | 15.34 | 2.40e-5 | -4.62 | 10.19 | 8.56e-4 | -3.07 |
| 2 | 0.999 | 28.73 | 14.1 | 5.68e-5 | -4.25 | 9.55 | 1.34e-3 | -2.87 | |
| 3 | 0.996 | 26.51 | 12.68 | 1.52e-4 | -3.82 | 8.09 | 3.67e-3 | -2.44 | |
| 53day-silique | 1 | 1.000 | 28.49 | 8.65 | 2.50e-3 | -2.6 | 4.7 | 3.86e-2 | -1.41 |
| 2 | 1.000 | 29.67 | 10.38 | 7.51e-4 | -3.12 | 6.67 | 9.79e-3 | -2.01 | |
| 3 | 1.000 | 27.85 | 8.88 | 2.13e-3 | -2.67 | 5.14 | 2.83e-2 | -1.55 | |
NGS expression data
Expression of miR395c transcript in NGS dataSequences
miRNA transcript
UCAUGACAGAGCAAGAAGAAGAAAGUCAAAUGUCCACAUGAGUUCCCUUUAACGCUUCAUUGUUGAAUACUCAAAGCCACAUUGGUUUGUAUAUAACACUGAAGUGUUUGGGGGGACUCUUGGUGUCAUUCUGGCGUUGAAGUGGCCACCAUUGCUGCGGUUUUCUAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CUGAAGUGUUUGGGGGGACUC | miRNA | 99 | 119 | |
TCATGACAGAGCAAGAAGAAGAA | primer | 5p | 1 | 23 |
ATAGAAAACCGCAGCAATGG | primer | 3p | 149 | 168 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR395c found in PubMed |