Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.53944 | 0.539984 | 5.61196 | 0.566386 | |
| 10day-seedling | 2.4745 | 0.630626 | 4.28266 | 0.734615 | |
| 14day-seedling | 1.37244 | 0 | 3.37353 | 0 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | 3.41576 | 0 | 5.26573 | 0 | |
| 42day-rosette | 3.63014 | 0 | 5.30611 | 0 | 42day-stem | 2.24825 | 0.547419 | 3.88271 | 0.496572 |
| 53day-rosette | 5.78287 | 5.08955 | 7.62537 | 5.04947 | 53day-stem | 3.9453 | 0.903993 | 5.37113 | 0.145145 | 53day-inflores | 2.49619 | 1.61873 | 4.16464 | 1.21338 | 53day-silique | 5.18203 | 0.197779 | 6.32463 | 0.237502 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 33.4 | 9.55 | 1.34e-3 | -2.87 | 9.46 | 1.42e-3 | -2.85 |
| 2 | 1.000 | 41.06 | 13.08 | 1.16e-4 | -3.94 | 13.21 | 1.05e-4 | -3.98 | |
| 3 | 1.000 | 36.13 | 11.86 | 2.69e-4 | -3.57 | 11.09 | 4.58e-4 | -3.34 | |
| 10day-seedling | 1 | 1.000 | 33.74 | 20.88 | 5.18e-7 | -6.29 | 15.39 | 2.33e-5 | -4.63 |
| 2 | 1.000 | 37.3 | 24.05 | 5.74e-8 | -7.24 | 18.24 | 3.23e-6 | -5.49 | |
| 3 | 1.000 | 31.47 | 20.1 | 8.91e-7 | -6.05 | 13.38 | 9.35e-5 | -4.03 | |
| 14day-seedling | 1 | 0.000 | 38.83 | 25.26 | 2.50e-8 | -7.6 | 18.61 | 2.50e-6 | -5.6 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 31.26 | 18.54 | 2.63e-6 | -5.58 | 12.4 | 1.84e-4 | -3.73 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.999 | 31.36 | 17.84 | 4.27e-6 | -5.37 | 12.27 | 2.02e-4 | -3.69 | |
| 42day-stem | 1 | 0.998 | 32.83 | 21.14 | 4.32e-7 | -6.36 | 15.83 | 1.71e-1 | -4.77 |
| 2 | 0.996 | 36.26 | 23.71 | 7.26e-1 | -7.14 | 18.17 | 3.48e-1 | -5.47 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | 1.000 | 5.24 | -8.81 | 4.48e+2 | 2.65 | -14.8 | 2.84e+4 | 4.45 |
| 2 | 1.000 | 31.8 | 19.51 | 1.34e-6 | -5.87 | 13.79 | 7.04e-5 | -4.15 | |
| 3 | 1.000 | 34.25 | 21.36 | 3.72e-7 | -6.43 | 14.7 | 3.76e-5 | -4.43 | |
| 53day-stem | 1 | 0.997 | 32.34 | 18.91 | 2.02e-6 | -5.69 | 12.4 | 1.86e-4 | -3.73 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.998 | 32.27 | 14.67 | 3.84e-5 | -4.42 | 11.71 | 2.98e-4 | -3.53 | |
| 53day-inflores | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.995 | 38.1 | 23.47 | 8.61e-8 | -7.06 | 18.91 | 2.03e-6 | -5.69 | |
| 3 | 0.998 | 31.63 | 17.8 | 4.37e-6 | -5.36 | 13.21 | 1.05e-4 | -3.98 | |
| 53day-silique | 1 | 1.000 | 31.78 | 11.93 | 2.56e-4 | -3.59 | 7.98 | 3.96e-3 | -2.4 |
| 2 | 1.000 | 32.27 | 12.98 | 1.24e-4 | -3.91 | 9.27 | 1.62e-3 | -2.79 | |
| 3 | 1.000 | 32.11 | 13.14 | 1.11e-4 | -3.96 | 9.41 | 1.47e-3 | -2.83 | |
NGS expression data
Expression of miR169h transcript in NGS dataSequences
miRNA transcript
AUUGUGUCCACUCCAUCUUCGUACGGUCAUGCCUAUAUAACACACAUAGUAGUCUUGUGGGAUACUCAUCAACAACCUCUUCAUAAAUACAUAAAUCAUAUAAGAGAAAAUGGUGACAUGAAGAAUGAGAACUUGUGUGGUAGCCAAGGAUGACUUGCCUGCGUUUUAGACCAUAUAUAUCAAAGACUCACUCGAUCGAUAGUCUUAGAGUUGGUUGGUCGUCAGGCAGUCUCCUUGGCUAUUCAAACAAUUCUCAUUCUCUUCAUUCACAUUUCUCUUUUUUGG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAGCCAAGGAUGACUUGCCUG | miRNA | 141 | 161 | |
TTGTGTCCACTCCATCTTCG | primer | 5p | 2 | 21 |
CCTGACGACCAACCAACTCT | primer | 3p | 207 | 226 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169h found in PubMed |